FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL
(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170
keywords: Daturametel, hepatocyte, inflammation, liver, photomicrograph
This study investigated the effects of ethanolic extracts of Daturametel parts on liver function of male albino rats. The plant parts are used for several reasons, including its medicinal and psychoactive activities. Thirty-five albino rats were used and distributed into 7 groups of 5 rats each. The extracts were administered to the test animals for 7 days before being sacrificed and blood collected for biochemical analysis. The serum activities of AST and ALT significantly (p<0.05) increased in all test groups, except (AST) in Group 4 (compared with Group 1). In Groups 3, 4, 5 and 7, ALP decreased significantly, but increased non-significantly in Groups 2 and 6 compared to the control. Total protein increased non-significantly (p>0.05) in Groups 2, 4, 5 and 6, but reduced non-significantly in Groups 3 and 7. Total bilirubin and indirect bilirubin increased significantly (p<0.05) in all groups administered the extracts. Direct bilirubin increased significantly (p<0.05) in Group 2 and 6, but increased non-significantly in Groups 3, 4, 5 and 7. Photomicrographs of some of the liver sections from rats administered the extracts show evidence of inflammatory cellular infiltration within the central canal and centrilobular hepatocytes, central canal rupture, degenerating hepatocytes, vacuolations, perivascular necrosis and widened sinusoids. However, portal tract and periportal hepatocytes appear fairly intact. These results show that despite the acclaimed medicinal effects of different parts of Daturametel, it can cause liver toxicity and may induce inflammatory cellular infiltration within some regions of the liver.
Abdullahi M, Muhammad G &Abdulkadir NU 2003. Medicinal and Economic Plants of Nupe land. Jube Evans Book and Publications, Nigeria, p. 234. Akinmoladun AC, AbukunEO, Afor E, Akinrinlola BL, OnibonTR, Akinboboye O, ObuotorEM&FarombiEO 2007. Chemical constituents and antioxidant activity of Alstoniaboonei.Afr. J. Biotech., 6: 1197-1201. Imo C &Uhegbu FO 2015.Phytochemical Analysis of GongronemalatifoliumBenth leaf using gas chromatographic flame ionization detector.Int. J. Chem. &Biomol. Sci., 1(2): 60-68. Imo C, Uhegbu FO, Imo CK&Ifeanacho NG 2013. Ameliorating effect and haematological activities of methanolic leaf extract of Gongronemalatifolium in acetaminophen induced hepatic toxicity in wistar albino rats. Int. J. Biosci., 3(11): 183-188. Khaton MM &Shaik MM 2012. Review on Daturametel: a potential medicinal plant. GJRMI., 1(4): 123–132. Ko RJ 1999. Causes, epidemiology, and clinical evaluation of suspected herbal poisoning. Clin.Toxicol., 37(6): 697–708. Kutama AS, Mohammed AS &Kiyawa SA 2010.Hallucinogenic effect of DaturametelL. leaf extract in albino rats.Biosci. Res. Communi., 22(4): 215-220. Manas KM, Pratyusha B &Debjani N 2012. Phytochemicals–biomolecules for prevention and treatment of human diseases: A review. Int. J. Scientific & Engr. Res., 3(7): 1-32. Moore M, Thor H, Moore G, Nelson S, Moldeus P &Orrenius S 1985. The toxicity of acetaminophen and N-acetyl p-benzoquinoneimine in isolated hepatocytes is associated with the depletion and increased cystosolicCa2+. J. Bio. Chem., 260: 13035-13040. Nair SP 2006. Protective effect of Tefroli- a polyherbal mixture (tonic) on cadmium chloride induced hepatotoxic rats. Pharmacognosy Magazine, 2(6): 112-118. Nuhu H 2002.Alakaloid content of the leaves of three Nigerian Datura species.Nig. J. Nat. Prod. & Med., 6: 15–18. RehamAM,Reham SR &Lamiaa AA 2009. Effect of substituting pumpkin seed protein isolate for caseinon serum liver enzymes, lipid profile and antioxidant enzymes in CCl4-intoxicated rats.Adv. Biol. Res., 3(1-2): 9-15. Sturgill MG & Lambert GH 1997.Xenobiotics induced hepatotoxicity: Mechanism of liver injury and method of monitoring hepatic function. Clin.Chem., 43: 1512- 1526. Yusuf M, Begum J, Hoque MN &ChowdhuryJU 2009. Medicinal plants of Bangladesh. BCSIR Chittagong, Bangladesh, p. 794.
